Nevan J. Krogan, PhD

Senior Investigator

Phone: (415) 476-2980
Fax: (415) 514-9736
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Other Professional Titles

Associate Professor, Cellular and Molecular Pharmacology, California Institute for Quantitative Biomedical Research, University of California, San Francisco

Administrative Assistant

Daryl Jones
(415) 734-2713
djones@gladstone.ucsf.edu

More about Dr. Krogan

Dr. Nevan Krogan is an Associate Investigator at both the Gladstone Institute of Virology and Immunology and the Gladstone Institute of Cardiovascular Disease. He is also an Associate Professor of Cellular and Molecular Pharmacology at the California Institute for Quantitative Biomedical Research at the University of California, San Francisco (UCSF).

Dr. Krogan’s research group develops and uses tools, such as genetic “maps,” to explore the inner workings of cells, including the multitude of proteins and genes and their relationships to each other. In addition, they use these tools to understand how infectious diseases, including HIV, tuberculosis and dengue, change the functioning of the cells they infect to replicate and spread. Understanding how the disease organisms hijack normal cellular processes can help in the development of targeted therapies to prevent or stem infections.

In 2009, Dr. Krogan was named a Distinguished Young Scholar by the W. M. Keck Foundation. His other awards and honors include the Hannah Farkas-Himsley and Alexander Memorial Award, the L.W. Macpherson Microbiology Award and the Searle Scholar Award.  In 2008, ScienceWatch.com named Dr. Krogan among the Top 25 authors of the most high-impact research papers in the fields of molecular biology and genetics. 

Originally from Canada, Dr. Krogan earned a bachelor’s degree in chemistry and a master’s degree in biochemistry from the University of Regina, in the Canadian province of Saskatchewan. He earned a PhD in medical genetics from the University of Toronto.

More scientific details, please

Other Professional Titles

Associate Professor, Cellular and Molecular Pharmacology, California Institute for Quantitative Biomedical Research, University of California, San Francisco

Administrative Assistant

Daryl Jones
(415) 734-2713
djones@gladstone.ucsf.edu

Areas of Investigation

The research in our laboratory focuses on the development of tools that allows for the generation, analysis and visualization of large-scale, quantitative genetic and physical interaction maps with the ultimate goal of further understanding cell physiology. Our group is presently interrogating a variety of biological processes in different eukaryotic (e.g. yeast, mouse, human) and prokaryotic (e.g. E. coli, B. subtilis, S. typhi) organisms. We are also interested in how these networks change under different conditions in a given organism as well as across different species. Furthermore, our group is also using these global, unbiased systems approaches to understand how HIV (and other pathogens) rewires the host’s cellular machinery during the course of infection. Ultimately, we predict that common host pathways and complexes will be targeted by multiple pathogens, and these will serve as better therapeutic targets for future studies.

We recently used a systematic affinity tag/purification-mass spectrometry (AP-MS) approach to purify all 18 HIV-1 polyproteins and processed proteins from two different cell types (HEK293 and Jurkat) and characterized the HIV–human complexes by mass spectrometry. Using a novel scoring algorithm we developed that quantitatively reports on mass spectrometry-derived protein-protein interactions, termed MiST (mass spectrometry interaction statistics), we derived a set of 497 HIV-human protein-protein interactions involving 435 distinct human proteins. This work led to a number of mechanistic insights about the function of specific HIV proteins during the course of HIV infection.

We are using other systems approaches to gain a deeper understanding of the HIV infection process. These include analyzing global changes in post-translational modifications in the HIV-infected host proteome, performing genetic interaction analyses with RNAi and mutant viruses. Finally, we are using these platforms to interrogate other pathogenic organisms and how they infect their host, including hepatitis C, influenza and West Nile virus.

Current Lab Focus

  • How do biological networks change under different stress conditions in a given organism and how do they evolve across different species?
  • What global changes can be seen in post-translational modification in the host proteome infected by HIV?
  • What genetic interactions exist between RNAi and mutant viruses?
  • Will hepatitis C, influenza and West Nile virus protein interactions with human proteins reflect similar or differing methods of host infection when compared to HIV?

Joined Gladstone

2011

Why Gladstone?

I am very excited to be part of both the Gladstone Institutes and QB3@UCSF. I feel this is a unique opportunity to utilize the systems approaches that are being developed at QB3@UCSF in collaboration with many scientists at the Gladstone Institutes to study important biomedical problems, including specific cardiovascular disease states and infection by pathogens such as HIV and HCV.  

Key Achievements

  • Developed computational and experimental tools for the functional interrogation of different biological systems using protein-protein and genetic interaction mapping approaches.
  • Deepened our understanding of how HIV proteins function during the course of infection by identifying several hundred host-pathogen protein interactions, most of which had not been previously identified.
  • Uncovered a new subunit of the Vif/Cul5 protein complex, called CBFß, that targets the APOBEC3 family of enzymes, restriction factors that inhibit HIV infection, for ubiquitination and degradation.

Education

University of Regina, Canada (BSc), Chemistry, High Honors (1997)
University of Toronto (PhD), Medical Genetics (2005)

Affiliations

Faculty, Chemistry and Chemical Biology Program, UCSF
Faculty, TETRAD Biochemistry and Molecular Biology Program, UCSF
Faculty, California Institute for Quantitative Biomedical Sciences, UCSF
Faculty, Integrative Program in Quantitative Biology Program, UCSF
Faculty, Cancer Genetics and Cell Cycling & Signaling, Helen Diller Family Comprehensive Cancer Center, UCSF
Faculty, Gladstone Institute of Cardiovascular Disease, The Gladstone Institutes
Faculty, Gladstone Institute of Virology and Immunology, The Gladstone Institutes

Awards

  • Canadian Cancer Society Studentship (1995)
  • Society of Chemical Industry Merit Award (1997)
  • Natural Sciences and Engineering Research Council PGS-B Award (1999)
  • Canadian Institute of Health Research (CIHR) Doctoral Fellowship (2001)
  • Hannah Farkas-Himsley and Alexander Memorial Award (2004)
  • L.W. Macpherson Microbiology Award (2005)
  • Searle Scholar, Searle Foundation (2009)
  • Keck Distinguished Young Scholar, W.M. Keck Foundation (2009)
  • Alumni Crowning Achievement Award, University of Regina (2012)
  • Cell Journal Top 40 under 40 (2014)
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